I compiled PyISAPIe on Python 2.7.2, 64 bit I have test it myself and it is working. If also need to use pyodbc in project, download and use this link " pyodbc-3.0.5 amd64 " otherwise on most other versions you will get ImportError. Instruction on how to deploy django on IIS can be found on this post . download: PyISAPIe-amd64-py2.7.2
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